Research Articles
Integrating PK-PD Modeling and Natural Product Research: From Complex Challenges to Clinical Translation
This article provides a comprehensive overview of the application of Pharmacokinetic-Pharmacodynamic (PK-PD) correlation modeling in natural product research.
Mastering the Glucose-Stimulated Insulin Secretion (GSIS) Assay for Stem Cell-Derived Beta Cells
The glucose-stimulated insulin secretion (GSIS) assay is the gold-standard functional test for evaluating stem cell-derived beta (SC-beta) cells, crucial for diabetes research and therapy development.
Ultracentrifugation Protocol for MSC Exosomes: A Complete Guide from Isolation to Characterization
This article provides a comprehensive guide for researchers and drug development professionals on isolating exosomes from Mesenchymal Stem Cell (MSC) conditioned media using ultracentrifugation.
Optimizing hiPSC Clinical Translation: Best Practices for Patient Recruitment and Robust Data Collection
This article provides a comprehensive guide for researchers and drug development professionals on establishing effective patient recruitment and clinical data collection frameworks for human induced pluripotent stem cell (hiPSC) studies.
Controlled Ice Nucleation: A Critical Parameter for Optimizing Cryopreservation Protocols and Post-Thaw Viability
This article provides a comprehensive analysis of ice nucleation temperature as a pivotal yet often overlooked factor in cryopreservation protocol optimization.
UMI Barcoding in Stem Cell scRNA-seq: A Complete Guide to Quantitative Analysis and Heterogeneity Resolution
Single-cell RNA sequencing (scRNA-seq) with Unique Molecular Identifiers (UMIs) has become an indispensable tool for dissecting the complex heterogeneity of stem cell populations, tracing lineage commitment, and understanding the molecular...
Vascular Integration in 3D Bioprinting: Strategies for Connecting Engineered Tissues to the Host Circulatory System
This article provides a comprehensive analysis of the strategies and challenges in achieving functional integration between bioprinted tissues and the host vasculature—a critical hurdle in regenerative medicine.
Epigenetic Remodeling in mRNA Reprogramming: Mechanisms, Therapeutics, and Clinical Translation
This article explores the convergence of epigenetic remodeling and mRNA technology for cellular reprogramming, a frontier in regenerative medicine and drug development.
Leveraging SSEA-1 Positive Cells for Enhanced Paracrine Factor Production in Embryonic Stem Cell Cultures
This article explores the strategic targeting of Stage-Specific Embryonic Antigen-1 (SSEA-1) positive cells within embryonic stem cell (ESC) cultures to amplify the yield and efficacy of paracrine mediators for therapeutic...
Paracrine Factors for Cytoprotection and Neovascularization: Mechanisms, Applications, and Therapeutic Frontiers
This article comprehensively explores the pivotal role of paracrine factors—biologically active molecules secreted by stem cells, particularly Mesenchymal Stem Cells (MSCs)—in mediating cytoprotection and neovascularization for cardiac repair.